Gene Edit Off Target Analysis Tool

Built upon BioEngine Technology

Advances in gene editing technology, in particular the CRISPR/Cas9 chemistry, have revolutionized gene editing with promise for advances in basic science, biotechnology, and biomedical research. With the simplicity of modern gene editing technologies come problematic off target interactions which can lead to failed experiments or cell death.

KBioBox's Gene Edit Off Target Analysis Tool was built to predict potential off targets, allowing scientists to redesign a target, or "know where to look" after a gene edit has been affected. Able to search entire genomes in under a minute, and customizable to any chemistry or enzyme protocol, the Gene Edit Off Target Analysis tool will provide a level of quality assurance previously unavailable for gene edits.


Simple to Use

3 Clicks for a Full Genome Analysis

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3 Click Submit

Enter cell line, gene edit type, and design, then the Off Target Analysis application built on the BioEngine takes over.

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Under a minute

Powered by the BioEngine, searching a whole genome for off-targets takes less than a minute, even for complex chemistries or enzymes

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Annotated Reports

Easy to understand reports, with intuitive visualizations and all locus informtaion provided

KBioBox's CEO introduces the Off Target Analysis System


BioEngine Results versus GUIDESeq

Outperforming Open Source Free Tools

KBioBox's Off Target Analysis application predicted 96.6% of off-targets found by GUIDE-seq, the MIT and E-CRISP tools found less then 50%.

On average KBioBox's Off Target Analysis ran in under two minutes, less than half the time for the MIT and E-CRISP tools.

Built upon KBioBox's BioEngine, the Off-Target Analysis tool predicted 96.6% of off-targets reported by the GUIDE-seq method in under two minutes. By comparison MIT's CRISPR design tool, and the E-CRISP tool both found less than 50% of total off-targets reported by GUIDE-seq.


Fully Customizable

Unique Enzymes/Chemistries

The Off Target Analysis application can be setup for any enzyme, chemistry, or custom reference.

Custom Reports

In need of additional information, or cross referencing between reports? Reports can be tuned to specific needs

Internal Access

Add the Off Target Analysis application to existing protocols, either through KBioBox's REST based API, or direct integration.